Dynamin 1 anticorps (pSer774)

N° du produit ABIN361464

Cet anticorps anti-Dynamin 1 est un anticorps Mouton Polyclonal détectant Dynamin 1 dans WB, IHC et IF. Adapté pour Rat.

Aperçu rapide pour Dynamin 1 anticorps (pSer774) (ABIN361464)

Antigène: Dynamin 1 (DNM1)

Reactivité: Rat

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp Dynamin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-Dynamin 1 anticorps

Épitope: pSer774

Specificité: Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by (-phosphatase treatment.

Réactivité croisée: Rat (Rattus)

Homologie: bovine, canine, chicken, human, mouse, non-human primates

Purification: Antigen Affinity Purified from Pooled Serum

Immunogène: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH

Information d'application

Indications d'application: Recommended Dilution: WB: 1:1000 IF (fixed cultured neurons): 1:1000 IHC (fixed cultured neurons): 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Stock: -20 °C

Détails sur Dynamin 1

Antigène: Dynamin 1 (DNM1)

Autre désignation: DNM1

Sujet: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking.

Poids moléculaire: '95 kDa

ID gène: 140694

UniProt: P21575

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling